Journal article
Intrafemoral Delivery of Hematopoietic Progenitors
M Evrard, I Kwok, LG Ng
Methods in Molecular Biology | HUMANA PRESS INC | Published : 2021
Abstract
Hematopoiesis is a central process and is essential for the replenishment of short-lived leukocytes such as neutrophils. However, the molecular events underlining the developmental transition of quiescent hematopoietic stem cells into downstream progenitors and mature blood cells are not completely understood. Here, we describe the intrafemoral delivery of hematopoietic progenitors as a method to trace their development and differentiation lineage patterns within the bone marrow (BM) niche. Unlike other approaches, the direct adoptive transfer of progenitors into the BM cavity does not require prior irradiation preconditioning of recipient mice, and enables the delivery of lower cell numbers..
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Awarded by Australian Research Council
Funding Acknowledgements
We thank the SIgN (Singapore Immunology Network) flow cytometry team for their technical assistance and support. This work was funded by the SIgN core funding, A*STAR (Agency for Science, Technology and Research), Singapore. M.E. is currently supported by the University of Melbourne McKenzie Postdoctoral Fellowship and grant DP200102753 from the Australian Research Council (ARC). The authors declare no conflict of interests. L.G.N. is supported by SIgN core funding. SIgN Flow Cytometry facility is supported by National Research Foundation (NRF) Singapore under Shared Infrastructure Support (SIS) (NRF2017_SISFP09).